Amulyaratna Behera 
,
Sunit Kumar Sahoo
For correspondence:- Amulyaratna Behera Email: amulyaslmp@rediffmail.com
Received: 23 July 2011 Accepted: 16 March 2012 Published: 15 June 2012
Citation: Behera A, Sahoo SK. Preparation and Evaluation of Glibenclamide-Loaded Biodegradable Nanoparticles. Trop J Pharm Res 2012; 11(3):345-350 doi: 10.4314/tjpr.v11i3.2
© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To formulate and evaluate glibenclamide (GB)-loaded poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) for controlled release.
Methods: GB-loaded PLGA NPs were prepared by solvent evaporation technique using methanol/dichloromethane (2:1) and characterized by transmission electron microscopy (TEM), and differential scanning calorimetry (DSC). The effect of stirring speed (250, 1000, 1500 and 2500 rpm) and drug: polymer ratio (1:1, 1: 2, 1:3 and 2:1) on particle size, size distribution, zeta potential, drug loading, encapsulation efficiency and drug release was also studied.
Results: Stable NPs were successfully prepared without any incompatibility, as indicated by TEM and DSC studies, respectively. As polymer and drug concentrations, and stirring speed increased, particle size, drug loading and encapsulation efficiency also increased. Increase in polymer concentration sustained drug release but reverse was obtained as drug concentration increased.
Conclusion: Controlled release biodegradable glibenclamide NPs can be efficiently prepared by emulsification solvent evaporation method suitably modulating processing variables.
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